![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K3292001:Design
Designed by: Abril Diosdado Hernandez, Gerardo David Diaz Renovado Group: iGEM19_Tec-Monterrey (2019-10-16)
T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The purification of the whole construct presented some disadvantages when using imidazole.
Source
Promoter Bba_K2406020 RBS Bba_J34801 Terminator Bba_B0010 sfGFP BBa_I746916 The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI))