Coding

Part:BBa_K2960007

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 00:47, 17 October 2019 by Registry (Talk | contribs)

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The mlrB serine protease

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.

MlrB is the second enzyme in the microcystin-degradation pathway. MlrB is a serine protease that catalyzes the hydrolysis of linearized MCs via the cutting of the peptide bond between alanine at position 1 and a variable amino acid (e.g. leucine) residue at position 2. This results in the formation of a tetrapeptide that is subsequently degraded by MlrC.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1124


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