Composite
MermAID

Part:BBa_K3280007

Designed by: Amanda Araujo Silva ; Raquel González Sterman ; Luana Corsi Antonio ; Juliana Naomi Yamauti Costa ; Nayla Naomi Kusimoto Takeuti ; Vinicius El Khalili Borsato   Group: iGEM19_USP_SaoCarlos-Brazil   (2019-10-16)


MermAID (Mercury binding peptide + CBD anchor + HlyA + tDGC)

This is a composite part. We call this part an “Iara alpha*â€. This Biobrick has a bidirectional promoter of MerR, a dCBD anchor, a lead binding peptide (MBP), a HlyA tag to secretion and a tDGC to induce biofilm formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2062
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1563


[edit]
Categories
Parameters
None