DNA

Part:BBa_K2919000:Design

Designed by: Evan Harrison   Group: iGEM19_MichiganState   (2019-10-04)
Revision as of 23:57, 16 October 2019 by Harr1335 (Talk | contribs) (→‎Design Notes)

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ghoT toxin from E. coli K12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A cumate inducible promoter, CuO, was used for expression, so that the bacteria could grow without the toxin being expressed and killing them off.

Source

This part comes from the E. coli K12 genomic sequence

References