Composite

Part:BBa_K3196034

Designed by: XinRan Rao   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 23:54, 16 October 2019 by Registry (Talk | contribs)

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Pgpda-GFP

Pgpda is a promoter amplified from the genome of Aspergillus niger, which can achieve maximum response at around pH5.0 and express the corresponding coding sequence. We can test the function of the promoter by measuring the fluorescence intensity at different pH gradients and get the related parameters. After testing the promoter, replace the GFP protein with the target gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 207
    Illegal BamHI site found at 850
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None