Coding

Part:BBa_K3165014:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)
Revision as of 23:35, 16 October 2019 by Registry (Talk | contribs)

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ccdB (L83S)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

The part is codon optimised for E. coli and all RFC compatible.


Source

The coding sequence is derived from Professor Raghavan Varadarajan's Lab at the Molecular Biophysics Unit in the Indian Institute of Science

References