Coding

Part:BBa_K3165013

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)
Revision as of 23:27, 16 October 2019 by Registry (Talk | contribs)

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i^2mCherry (Ile)

i^2mCherry was developed to limit the truncation caused by the existing mCherry sequence in the iGEM registry. To reduce the truncation to the minimum possible value we replace the internal start codon (ATG) to ATC coding for Isoleucine. It results from a single base mutation at the 48th base pair.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


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