![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3258017
Designed by: Alec Lourenco Group: iGEM19_Stanford (2019-10-16)
Self-amplifying fluorescent RNA (MDV-Q-beta-Spinach)
This is a part composite composed of self-encoded Qbeta replicase with a spinach aptamer attached, both sequences flanked by midivariant regions recognized by Qbeta. When expressed in E. coli cell free extract supplemented with DFHBI, Spinach fluorescence increases due to RNA replication.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 180
Illegal XbaI site found at 2231
Illegal SpeI site found at 2107 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 146
Illegal SpeI site found at 2107
Illegal NotI site found at 2213 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 172
Illegal BglII site found at 2228
Illegal BamHI site found at 1538
Illegal BamHI site found at 1681
Illegal XhoI site found at 168
Illegal XhoI site found at 1212
Illegal XhoI site found at 2222 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 180
Illegal XbaI site found at 2231
Illegal SpeI site found at 2107 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 180
Illegal XbaI site found at 2231
Illegal SpeI site found at 2107
Illegal NgoMIV site found at 2120
Illegal NgoMIV site found at 2149
Illegal AgeI site found at 1694 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |