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Tag
Part:BBa_K3258016
Designed by: Alec Lourenco Group: iGEM19_Stanford (2019-10-16)
Downstream MDV
Midivariant RNA (MDV) is RNA that is readily recognized and replicated by Q-beta replicase, an RNA-dependent RNA polymerase. To increase the replicability of RNA by Q-beta replicase, the MDV can be split into sub-parts and added upstream and downstream of the RNA of interest. This is the downstream complementary DNA that should be added to the cDNA of interest.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 23
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 5
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
Illegal XhoI site found at 14 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 23
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 23
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |