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Part:BBa_K3258015

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)
Revision as of 22:47, 16 October 2019 by Registry (Talk | contribs)

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Upstream MDV

Midivariant RNA (MDV) is RNA that is readily recognized and replicated by Q-beta replicase, an RNA-dependent RNA polymerase. To increase the replicability of RNA by Q-beta replicase, the MDV can be split into sub-parts and added upstream and downstream of the RNA of interest. This is the upstream complementary DNA that should be added to the cDNA of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal XhoI site found at 168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


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