RNA

Part:BBa_K3081104:Design

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 22:29, 16 October 2019 by Lelezhang (Talk | contribs) (→‎Design Notes)


scaffold of sgRNA in CRISPR/Cas system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed.

Source

E.coli

References

Previous publication: Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v