Coding

Part:BBa_K2984051

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 20:30, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


L1c-PsaD-bleRscp-YFP-RbcS2

This part is composed of the PsaD promoter (BBa_K2984008), a bleomycin resistance with self cleaving peptide scp (BBa_K2984045), a B2-B2 linker (BBa_K2984034), a YFP mVenus marker (BBa_K2984017), and the Rbcs2 terminator (BBa_K2984018).

This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1833
    Illegal NgoMIV site found at 1453
    Illegal NgoMIV site found at 2310
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None