Composite

Part:BBa_K3149001

Designed by: Triana Mayra Sánchez Huerta   Group: iGEM19_TecMonterrey_GDL   (2019-10-16)
Revision as of 20:08, 16 October 2019 by Registry (Talk | contribs)

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TRZ1 receptor under the control of the pOmpC promoter with iLov fluoresent reporter

Safety and chemicals Aside from microorganisms, we used several chemical reagents, one being ethidium bromide (EtBr). During our experimental procedures, we worked with this reagent to visualize DNA during electrophoresis, and it is believed to be a mutagen and probable carcinogen.

As an alternative, we employed GelRed, an alternative marker for DNA electrophoresis. GelRed is marked as less toxic and more sensitive than ethidium bromide (EtBr), becoming a priority during these procedures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
    Illegal AgeI site found at 802
    Illegal AgeI site found at 1351
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None