![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3149001
Designed by: Triana Mayra Sánchez Huerta Group: iGEM19_TecMonterrey_GDL (2019-10-16)
TRZ1 receptor under the control of the pOmpC promoter with iLov fluoresent reporter
Safety and chemicals Aside from microorganisms, we used several chemical reagents, one being ethidium bromide (EtBr). During our experimental procedures, we worked with this reagent to visualize DNA during electrophoresis, and it is believed to be a mutagen and probable carcinogen.
As an alternative, we employed GelRed, an alternative marker for DNA electrophoresis. GelRed is marked as less toxic and more sensitive than ethidium bromide (EtBr), becoming a priority during these procedures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 690
Illegal AgeI site found at 802
Illegal AgeI site found at 1351 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |