Generator

Part:BBa_K1741014:Experience

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 20:06, 16 October 2019 by Goktugattar (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1741014

Bilkent-UNAMBG 2019 iGEM Team

We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.

T--Bilkent-UNAMBG--PZE.png


Figure 1:


T--Bilkent-UNAMBG--PZA.png


Figure 2:


T--Bilkent-UNAMBG--PZS.png


Figure 3:


T--Bilkent-UNAMBG-PZSOD.png


Figure 4:


T--Bilkent-UNAMBG-PZAOD.png


Figure 5:


T--Bilkent-UNAMBG-PZEOD.png


Figure 6:

User Reviews

UNIQ2e540ddb03e3fc1f-partinfo-00000000-QINU UNIQ2e540ddb03e3fc1f-partinfo-00000001-QINU