Regulatory

Part:BBa_K3222000

Designed by: Ryo NIWA   Group: iGEM19_GIFU_TOKAI   (2019-10-16)
Revision as of 19:56, 16 October 2019 by Registry (Talk | contribs)

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T7 promoter with GGG of 3' end

BBa_K3222000 is an improved part of BBa_I746916 which encodes super folder GFP gene (Pedelacq et al. (2006): "Engineering and characterization of a super folder green fluorescent protein", Nature Biotech 24 (1) January 2006). We changed 15A to G to remove Sap1 restriction site with the silent mutation. This part meets both Biobrick standard and Type llS standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None