Coding

Part:BBa_K2984048

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 19:46, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


L1c-PsaD-bleRscp-ARS-PETase-SP20-RbcS2

This part is composed of the PsaD promoter (BBa_K2984008), bleomycin resistance with self cleaving peptide scp (BBa_K2984045), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), the secretion enhancing SP20 glycomodule (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every bleomycin resistance, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:1 to the antibiotic. By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1607
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None