Tag

Part:BBa_K3117005

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 19:32, 16 October 2019 by Lena Schorr (Talk | contribs)


6xHis-Tag

The part BBa_K3117005 is a 6xHis tag which can be used for protein purification and for the evidence of marked proteins. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None