Composite

Part:BBa_K3110043

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 19:08, 16 October 2019 by Suryazzz (Talk | contribs)

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J23117 Strong RBS sfGFP

There is constitutive Promoter (J23117) followed by Strong RBS (B0034) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Strong RBS sfGFP (BBa_K3110040) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 66


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Categories
Parameters
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