Regulatory

Part:BBa_K3032112:Design

Designed by: Gytis Druteika   Group: iGEM19_Vilnius-Lithuania   (2019-10-16)
Revision as of 18:51, 16 October 2019 by Gytisdruteika (Talk | contribs)

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EL222 regulated promoter based on Anderson (0.16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EL222 DNA binding sequence was carefully inserted into weak promoter’s region of -35 to -10 in order not to affect promoter's activity.


Source

This promoter sequence is design by us. EL222 DNA binding sequence (DOI: 10.1093/nar/gkw548) was inserted into weak promoter’s region of -35 to -10.


References