Regulatory

Part:BBa_K3002003

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 18:25, 16 October 2019 by Registry (Talk | contribs)

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pAR promoter for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the PAR promoter (A1-A3) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with an RBS, a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. As this part is a combination of the promoters HSP70A (upstream) and RBCS2 no heat shock is needed as a transcription activator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None