Composite
Part:BBa_K2971004:Design
Designed by: Simen Hermansen Group: iGEM19_UiOslo_Norway (2019-10-16)
crtEBI under pBad promotor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 4181 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2854
Illegal BamHI site found at 1144
Illegal BamHI site found at 2093 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1295
Illegal SapI site found at 961
Design Notes
Codon optimized for e.coli
Source
deinococcus radiodurans