Composite

Part:BBa_K2999010

Designed by: Xin Ma   Group: iGEM19_YAU-China   (2019-10-16)
Revision as of 18:15, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pPA2069+RBS+PrtN

PrtN, a lysozyme activating protein of Pseudomonas aeruginosa PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 365
    Illegal XhoI site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989


[edit]
Categories
Parameters
None