Coding

Part:BBa_K3007012

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)
Revision as of 18:04, 16 October 2019 by Registry (Talk | contribs)

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Humanized mammalian urate-dependent transsilencer (mUTS)

This part can expresses a protein fused by human KRAB (Krueppel-associated box) domain and a hypothetical uricase regulator (HucR). HucR works as a homo-dimer and binds to a special dyad-symmetrical operator site named hucO in the absence of UA with high affinity. The KRAB domain enhances the inhibition efficiency when HucR-hucO interaction exists.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 244
    Illegal SapI.rc site found at 208


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Parameters
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