![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3007012
Designed by: Cheng Li Group: iGEM19_QHFZ-China (2019-10-16)
Humanized mammalian urate-dependent transsilencer (mUTS)
This part can expresses a protein fused by human KRAB (Krueppel-associated box) domain and a hypothetical uricase regulator (HucR). HucR works as a homo-dimer and binds to a special dyad-symmetrical operator site named hucO in the absence of UA with high affinity. The KRAB domain enhances the inhibition efficiency when HucR-hucO interaction exists.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 508
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 244
Illegal SapI.rc site found at 208
[edit]
Categories
Parameters
None |