Composite

Part:BBa_K3105679

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)
Revision as of 18:01, 16 October 2019 by Tereza (Talk | contribs)


Expression construct HRP-2A-AAO

Expression circuit for HRP and AAO in Pichia pastoris. HRP will be secreted due to the addition of the α-factor secretion signal and AAO will remain in the cell. The 2A-selfcleaving peptide will lead to cleavage of the polypeptide, achieving separation of HRP from AAO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2086
    Illegal BamHI site found at 2481
    Illegal BamHI site found at 2668
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2167
    Illegal AgeI site found at 3628
    Illegal AgeI site found at 4241
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None