RBS

Part:BBa_K3059620

Designed by: Julia Urban   Group: iGEM19_William_and_Mary   (2019-10-16)
Revision as of 17:31, 16 October 2019 by Registry (Talk | contribs)

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pre-csgE RBS

RBS to follow csgBAC and precede csgEFG operon in a synthetic curli operon.


The native curli operon in MG1655 E. coli contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, both csgBAC and csgEFG are necessary (for example, though csgA is the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.

When successfully assembled in a transcriptional unit with a promoter, csgBAC, csgEFG, and a terminator, this RBS results in curli amyloid fiber production. Curli fibers are native to E. coli and contribute to biofilm formation and strength.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None