Coding

Part:BBa_K3081119

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 16:56, 16 October 2019 by Registry (Talk | contribs)

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dCas9-linker-sfGFP

In this part, sfGFP is fused to the C terminus of dCas9, and we use it to evaluate the expression of dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4147


[edit]
Categories
Parameters
None