Composite

Part:BBa_K3171172:Design

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-16)
Revision as of 16:46, 16 October 2019 by Registry (Talk | contribs)

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Vibrio natriegens Native P1 promotor with mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
    Illegal NotI site found at 13
    Illegal NotI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7
    Illegal XbaI site found at 22
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P1+mCherry fusion


Source

mCherry was obtained from the registry BBa_J06602. Native P1 promoter was obtained from IDT

References