Coding

Part:BBa_K3007031

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)
Revision as of 16:23, 16 October 2019 by Registry (Talk | contribs)

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Expression device of RinA_p80α under uric acid-responsive regulatory system

It is a UA concentration-responsive system in E. coli. CP6 promoter promotes the expression of HucR and YgfU constitutively. If the concentration of uric acid (UA) in environment is low, HucR will bind to PhucR, which inhibits the expression of downstream reporter, dsRed. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from PhucR, and induces the amplifier RinA_p80α expression to enhance the downstream gene expression.

This composite part is equal to BBa_K3007007, which is wrongly registered as a basic part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal NheI site found at 830
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal BglII site found at 597
    Illegal BamHI site found at 158
    Illegal BamHI site found at 2011
    Illegal BamHI site found at 2875
    Illegal BamHI site found at 2950
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
    Illegal AgeI site found at 1829
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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