![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K118024
dxs+LIMS1+appY
dxs, from Escherichia coli JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here]. lims1, from Citrus limon, encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway). (+)-Limonene is a component of lemon scent. appY, from Escherichia coli JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005). (All three genes are individually preceded by a ribosome binding site.)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 746
Illegal SapI.rc site found at 2006
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