Plasmid
Part:BBa_K3179106:Design
Designed by: Jiaxian Xu Group: iGEM19_SYSU-CHINA (2019-10-16)
pT2mK2E
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2500
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3326
Illegal BamHI site found at 954
Illegal BamHI site found at 3578 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500
Illegal NgoMIV site found at 1224
Illegal NgoMIV site found at 2219
Illegal NgoMIV site found at 3848
Illegal AgeI site found at 960
Illegal AgeI site found at 3584 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3352
Design Notes
pTRE-mi663b-2Kt-E1-E1B55K-miR885-5p
Source
pTRE-mi663b-2Kt-E1-E1B55K-miR885-5p