Plasmid

Part:BBa_K3179106:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)
Revision as of 16:09, 16 October 2019 by Registry (Talk | contribs)

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pT2mK2E


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1750
    Illegal XbaI site found at 4374
    Illegal PstI site found at 2500
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3326
    Illegal BamHI site found at 954
    Illegal BamHI site found at 3578
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1750
    Illegal XbaI site found at 4374
    Illegal PstI site found at 2500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1750
    Illegal XbaI site found at 4374
    Illegal PstI site found at 2500
    Illegal NgoMIV site found at 1224
    Illegal NgoMIV site found at 2219
    Illegal NgoMIV site found at 3848
    Illegal AgeI site found at 960
    Illegal AgeI site found at 3584
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3352


Design Notes

pTRE-mi663b-2Kt-E1-E1B55K-miR885-5p


Source

pTRE-mi663b-2Kt-E1-E1B55K-miR885-5p

References