Measurement

Part:BBa_K118021

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-10-07)
Revision as of 13:43, 7 October 2008 by Registry (Talk | contribs)

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PcstA+rbs+xylE

xylE is from the Pseudomonas putida TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply has been added to the glucose-repressible promoter of cstA. This allows for characterisation of pCstA over a range of glucose concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 476
    Illegal NgoMIV site found at 648
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None