Coding
Part:BBa_K2449003
Designed by: Malte Skovsager Andersen Group: iGEM17_SDU-Denmark (2017-07-20)
Revision as of 15:36, 16 October 2019 by Willie Jin (Talk | contribs)
cep94A
This is the so called cellobiose phosphorylase (Cep94A), which phosphorylates the cellobiose β-linkage resulting in its breakdown to D-glucose and α-D-glucose-1-phosphate. It is used in the following biobrick BBa_K2449004 For more info visit https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294459/
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 846
Illegal NgoMIV site found at 1942 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 108
ECUST_China 2019 characterization
In ECUST_China 2019 characterization, we used Cep 94A to degrade cellobiose to glucose and glucose-phosphate. To characterize the Cep 94A specifically, we performed SDS-PAGE and measured enzyme activity.
SDS-PAGE revealed that Cep 94A was expressed successfully (about 91kDa). After that, we sonicated E.coli and extract Cep 94A, using DNS assay to measure the enzyme activity.
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Categories
Parameters
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