Device

Part:BBa_K3264023:Design

Designed by: Xinyou Chang   Group: iGEM19_GreatBay_SZ   (2019-10-16)
Revision as of 15:28, 16 October 2019 by Yoyoyo (Talk | contribs) (→‎Source)


TALEsp-vioA-vioB-vioC-vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

an C-terminal domain based on the E. australis MiSp1 sequence (VTSG GYGYGTSAAA GAGVAAGSYA GAVNRLSSAE AASRVSSNIA AIASGGASAL PSVISNIYSG VVASGVSSNE ALIQALLELL SALVHVLSSA SIGNVSSVGV DSTLNVVQDS VGQYVG) Also, the linker between the CT and the Rep (repetitive region) is GNS The construct was cloned into a pT7 plasmid and transformed into BL21 (DE3) E. coli bonded to NT-2Rep-CT


Source

References