Coding

Part:BBa_K3268000

Designed by: YI-HSUAN CHAN   Group: iGEM19_NYMU-Taipei   (2019-10-11)
Revision as of 15:10, 16 October 2019 by Nymuchang (Talk | contribs)


mOR103-15

This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.

mOR103-15
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834


[edit]
Categories
Parameters
None