Regulatory

Part:BBa_K3286042:Design

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 15:03, 16 October 2019 by SJK (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Native bacteriophage Lambda PL promoter, OL operators and 5' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To assess whether bacteriophage Lambda could be inhibited by CRISPRi, we required the native promoter with the OL operators and the 5'UTR.

Source

Sequence was obtained from NCBI Reference Sequence: NC_001416.1 and synthetically synthesised in a gBlock.

References