Regulatory

Part:BBa_K3286042:Design

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 15:01, 16 October 2019 by SJK (Talk | contribs) (→‎Source)


Native bacteriophage Lambda PL promoter, OL operators and 5' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To assess whether we could inhibit bacteriophage lambda, we required the native promoter.


Source

Sequence was obtained from NCBI Reference Sequence: NC_001416.1 and synthetically synthesised in a gBlock.

References