Coding

Part:BBa_K3007029

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)
Revision as of 14:54, 16 October 2019 by Registry (Talk | contribs)

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Expression device of dsRed under uric-acid-responsive regulatory system

It works as a uric acid reporter. Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to PhucR, which inhibits the expression of downstream reporter, dsRed. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from PhucR, and induces the fluorescent protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal NheI site found at 1085
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal BglII site found at 852
    Illegal BamHI site found at 158
    Illegal BamHI site found at 2266
    Illegal BamHI site found at 3130
    Illegal BamHI site found at 3205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
    Illegal AgeI site found at 2084
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
None