Coding

Part:BBa_K3007027

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)
Revision as of 14:33, 16 October 2019 by LeeOrange (Talk | contribs)


Reverse version of BBa_K3007025

Hypothetical uricase regulator (HucR) from D. radiodurans is a bacterial transcriptional factor, which acts as a homo-dimer and binds to hucO in the absence of uric acid. When uric acid is present, HucR dissociates from hucO, allowing expression of a downstream gene. YgfU is a transporter for importing uric acid into cytoplasm in E. coli. In order to simplify the experiment design, QHFZ-China 2019 added the sequence coding YgfU after the CDS of HucR under the control of a continuous promoter, Pcp6 promoter, which forms a new composite part to transport and detect the exocellular uric acid. The part is equal to BBa_K3007003, because of a mistake in part document.

It is a reverse version of BBa_K3007025, which is used to document a bigger composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 309
    Illegal BamHI site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 309
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None