Coding

Part:BBa_K118016:Design

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-10-06)
Revision as of 21:20, 6 October 2008 by Andhi (Talk | contribs) (→‎Design Notes)

glgC16 (glgC with G336D substitution)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) using the mutagenesis by blunt-ended ligation (MABEL) technique. The genomic DNA sequence also contains to EcoRI sites, both of which have been mutated out using MABEL.

Source

Escherichia coli JM109 genomic DNA

References