Coding

Part:BBa_K2936001:Design

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-08)
Revision as of 14:19, 16 October 2019 by Can can (Talk | contribs) (→‎Design Notes)

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pBAD-LacZɑ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When the BAD promoter was transcribed by RNA polymerase, the β-galactosidase encoded by LacZ alpha gene could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.

Source

The pGLO plasmid

References