Coding
chbR

Part:BBa_K3093000:Design

Designed by: Jin Qianwei   Group: iGEM19_ECUST_China   (2019-08-23)
Revision as of 14:17, 16 October 2019 by Still Water (Talk | contribs) (→‎Source)


chbR N238S Y30C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ChbR is encoded within the chb operon itself. In the presence of chitobiose, ChbR binds to two direct repeats located between two NagC binding sites and activates the transcription of the chb operon by overlapping the -35 box of chbBp. In the absence of chitobiose, ChbR acts as a repressor of the chb operon. Monoacetylchitobiose-6-P has been experimentally determined to be the inducer for ChbR.

Source

ChbR was obtained from the genomic sequence of Escherichia coli str. K-12 substr.MG1655

References