Regulatory

Part:BBa_K2936012:Design

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-09)
Revision as of 13:56, 16 October 2019 by Can can (Talk | contribs) (→‎Design Notes)

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araBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction.

Source

The pGLO plasmid

References