Regulatory
Part:BBa_K3286043
Designed by: Sebastiaan Kuiper Group: iGEM19_Wageningen_UR (2019-10-16)
Native bacteriophage Lambda PR and PRM promoter, OR operators and 5' UTR
Native bacteriophage Lambda PL promoter, upstream of the CI (Lambda repressor) protein.
The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |