Tag

Part:BBa_K3002018

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 13:29, 16 October 2019 by MSchlosser (Talk | contribs)


Sp20 8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the sp20 8xHis-tag (B5) and was built as a part of the Kaiser Collection. Combined with a secretion signal of the Kaiser Collection like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006(RPL23 terminator)) your target protein is secreted efficiently and can be purified via a nickel-NTA column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None