Coding
ADR CMYC 6

Part:BBa_K3038001:Design

Designed by: Marine LAVAUD   Group: iGEM19_Poitiers   (2019-10-10)
Revision as of 13:10, 16 October 2019 by Bvannier (Talk | contribs) (→‎References)


Alcohol dehydrogenase - Geobacillus stearothermophilus_Cmyc and 6His tags_Cterminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RFC10 standard has been included in the design. C Myc and 6his Tags have been added to the Cterminal part of the ADR sequence. GC content optimized for the synthesis.


Source

It comes from a synthetic gene, with the RFC10 standard, synthesized by IDT.


References

Gene structure and amino acid sequences of alcohol dehydrogenases of Bacillus stearothermophilus. Robinson,G.A., Bailey,C.J. and Dowds,B.C. JOURNAL Biochim. Biophys. Acta 1218 (3), 432-434 (1994)

Overexpression, Purification, and Biochemical Characterization of the Thermostable NAD-dependent Alcohol Dehydrogenase from Bacillus stearothermophilus. SHIM, EUN-JUNG, SANG-HOON JEON, AND KWANG-HOON KONG. J. Microbiol. Biotechnol. (2003), 13(5), 738–744