Coding
Part:BBa_K3040100:Hard Information
Designed by: Lai, Si-Ying Group: iGEM19_NTHU_Taiwan (2019-10-15)
Revision as of 13:04, 16 October 2019 by Liya (Talk | contribs) (Created page with "Lipase from Antarctic cold-tolerant Pseudomonas sp. 7323 can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of...")
Lipase from Antarctic cold-tolerant Pseudomonas sp. 7323 can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.