Part:BBa_K3205001

Pare promoter region

Pare is a AreR-regulated σ54-dependent promoter in Acinetobacter sp. Strain ADP1. are operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.

Usage and Biology

AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

For more details, please click the link below.

https://parts.igem.org/Part:BBa_K3205000