Part:BBa_K2984036

L1c-PsaD-PETase-YFP-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a HA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1915
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1244
  Illegal PstI site found at 1915
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal XhoI site found at 968
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1915
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1915
  Illegal NgoMIV site found at 2392
  
• 1000
  COMPATIBLE WITH RFC[1000]