Primer

Part:BBa_K2982008:Design

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-16)
Revision as of 12:46, 16 October 2019 by T ocean (Talk | contribs) (→‎Design Notes)

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Reverse primer for amplifying BBa_E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has a XhoI restriction site that allows the cutting of the part. It also contains a linkage sequence of the PET-28a vector between the RE site and the GFP gene.

Source

iGEM registry

References