Coding
Mlut11700

Part:BBa_K3038004:Design

Designed by: Anas CANTEAU   Group: iGEM19_Poitiers   (2019-10-15)
Revision as of 12:40, 16 October 2019 by Bvannier (Talk | contribs) (→‎References)


Acyl CoA oxydase : Mlut11700_N-ter C-myc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1682
    Illegal XhoI site found at 1925
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1816
    Illegal BsaI.rc site found at 2047
    Illegal SapI site found at 172


Design Notes

RFC10 standard GC content optimized


Source

Sequence synthezized by IDT


References

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610